0to300golf: December 2021

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So Jin's lab took a more global strategy. Klug, who's a analysis associate at Salk. Because the TRN outcome was so unexpected, Jin and first writer Jason Klug next sought to verify their initial rabies result with two independent methods: optogenetics, which makes use of gentle to stimulate neurons to fire, and electrophysiology, which information electrical exercise. The neurons did, which validated the evidence for TRN-striatal communication. Together, these bolstered the clinical observations in human patients and implied their different roles in cognitive versus sensorimotor operate. In doing so, the Salk staff discovered a number of fascinating things: first, that communication indicators to the ChAT neurons got here primarily from areas of the brain liable for cognitive features; and, second, that input to the PV neurons came mainly from sensorimotor areas. In addition, the team found a novel connection no one noticed earlier than: a cluster of neurons called the thalamic reticular nucleus (TRN) that project directly to the striatum. This was stunning because the TRN was thought to be limited in its projections to the brain area for which it is named, the thalamus. They used a method pioneered by fellow Salk scientist Edward Callaway to harness a modified rabies virus tagged with a fluorescent marker to trace which brain cells were speaking with each other in mice. While causing neurons in the TRN to fire, the researchers recorded whether PV interneurons in the striatum responded.